An important aspect of any biotechnological processes is the culture of animal cells in artificial media. These animal cells in culture are used in recombinant DNA technology, genetic manipulations and in a variety of industrial processes. Animal cells e.g. egg cells are used for multiplication of superior livestock using a variety of techniques like cloning of superior embryonic cells, transformation of cultured cells leading to the production of transgenic animals. The animal cells are also used in vitro fertilization and transfer of embryos to surrogate mothers. Hence the establishment and maintenance of a proper animal culture is the first step towards using them as tools for biotechnology.